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human prostate epithelial cells p69  (ATCC)


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    ATCC human prostate epithelial cells p69
    The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) <t>P69,</t> prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.
    Human Prostate Epithelial Cells P69, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate epithelial cells p69/product/ATCC
    Average 99 stars, based on 530 article reviews
    human prostate epithelial cells p69 - by Bioz Stars, 2026-06
    99/100 stars

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    1) Product Images from "Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction"

    Article Title: Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-13-1062

    The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) P69, prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.
    Figure Legend Snippet: The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) P69, prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.

    Techniques Used: Infection, Purification, Northern Blot, Control

    The indicated cell lines were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell lysates were collected. For detecting protein expression, 50 μg of total protein was run on SDS–PAGE, transferred onto a nitrocellulose membrane and stained with the indicated antibodies, and protein expression was determined by Western blotting in A) Im-PHFA, malignant glioma and neuroglioma, B) P69 and prostate cancer cells, and C) FM516 and melanomas. D) Cells were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell proliferation/viability was assayed by MTT assay. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 cell viability is significantly less than cells infected for 0 h (one way ANOVA with Newman Keuls).
    Figure Legend Snippet: The indicated cell lines were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell lysates were collected. For detecting protein expression, 50 μg of total protein was run on SDS–PAGE, transferred onto a nitrocellulose membrane and stained with the indicated antibodies, and protein expression was determined by Western blotting in A) Im-PHFA, malignant glioma and neuroglioma, B) P69 and prostate cancer cells, and C) FM516 and melanomas. D) Cells were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell proliferation/viability was assayed by MTT assay. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 cell viability is significantly less than cells infected for 0 h (one way ANOVA with Newman Keuls).

    Techniques Used: Infection, Expressing, SDS Page, Membrane, Staining, Western Blot, MTT Assay

    A) SARI full length promoter/pGL3-Luc or SARI deletion mutant promoter/PGL3-Luc constructs were transfected into HeLa cells. Twenty-four h later the cells were treated with IFN-β (1000 U/ml) or infected with Ad.vec (100 pfu/cell) or Ad.mda-7 (100 pfu/cell) for 24 h after which cell lysates were collected for luciferase assays. B) i): HeLa cells were infected with Ad.vec or Ad.SARI at 10 pfu/cell and nuclei were prepared from the treated cells. The isolated nuclei were used to label preinitiated RNA transcription with [α-32P] UTP in vitro, and the purified RNA was hybridized to a dot blot carrying an equivalent amount of a SARI cDNA. The transcription rate of GAPDH served as control. ii) HeLa cells were treated as described in panel A and total RNA was purified, blotted onto a nylon membrane, and Northern blotting was done with a SARI, mda-7/IL-24 or GAPDH cDNA. C) 3′-UTR region of SARI mRNA was cloned into PGL3-basic-Luc vector (Luc-3′UTR) and three independent clones (along with control vector) were transfected into HeLa cells for 24 h followed by infection with Ad.vec or Ad.mda-7 (100 pfu/cell for 24 h) and cell lysates were collected for luciferase assays. The data is presented as the ratio of Ad.mda-7 to Ad.vec treated cells. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 RLU is significantly higher than PGL-3 Luc control cells. D) P69 cells were infected with Ad.vec or Ad.mda-7 (20 pfu/cell for 24 h) after which Actinomycin D (0.5 μg/ml) was added for the indicated times. Total RNA was collected to perform Northern blotting using a SARI cDNA.
    Figure Legend Snippet: A) SARI full length promoter/pGL3-Luc or SARI deletion mutant promoter/PGL3-Luc constructs were transfected into HeLa cells. Twenty-four h later the cells were treated with IFN-β (1000 U/ml) or infected with Ad.vec (100 pfu/cell) or Ad.mda-7 (100 pfu/cell) for 24 h after which cell lysates were collected for luciferase assays. B) i): HeLa cells were infected with Ad.vec or Ad.SARI at 10 pfu/cell and nuclei were prepared from the treated cells. The isolated nuclei were used to label preinitiated RNA transcription with [α-32P] UTP in vitro, and the purified RNA was hybridized to a dot blot carrying an equivalent amount of a SARI cDNA. The transcription rate of GAPDH served as control. ii) HeLa cells were treated as described in panel A and total RNA was purified, blotted onto a nylon membrane, and Northern blotting was done with a SARI, mda-7/IL-24 or GAPDH cDNA. C) 3′-UTR region of SARI mRNA was cloned into PGL3-basic-Luc vector (Luc-3′UTR) and three independent clones (along with control vector) were transfected into HeLa cells for 24 h followed by infection with Ad.vec or Ad.mda-7 (100 pfu/cell for 24 h) and cell lysates were collected for luciferase assays. The data is presented as the ratio of Ad.mda-7 to Ad.vec treated cells. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 RLU is significantly higher than PGL-3 Luc control cells. D) P69 cells were infected with Ad.vec or Ad.mda-7 (20 pfu/cell for 24 h) after which Actinomycin D (0.5 μg/ml) was added for the indicated times. Total RNA was collected to perform Northern blotting using a SARI cDNA.

    Techniques Used: Mutagenesis, Construct, Transfection, Infection, Luciferase, Isolation, In Vitro, Purification, Dot Blot, Control, Membrane, Northern Blot, Clone Assay, Plasmid Preparation



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    human prostate epithelial cells p69  (ATCC)
    99
    ATCC human prostate epithelial cells p69
    The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) <t>P69,</t> prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.
    Human Prostate Epithelial Cells P69, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prostate epithelial cells p69/product/ATCC
    Average 99 stars, based on 1 article reviews
    human prostate epithelial cells p69 - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) P69, prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.

    Journal: Cancer research

    Article Title: Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction

    doi: 10.1158/0008-5472.CAN-13-1062

    Figure Lengend Snippet: The indicated cells were infected as indicated with Ad.vec or Ad.mda-7 (or Ad.p53) at 20 pfu/cell (HeLa, LNCaP) or 100 pfu/cell (other cells) for 24 h. For Quantitative mRNA detection, total RNA was purified, blotted onto nylon membranes and Northern blotting was done with a SARI cDNA. A) Im-PHFA, malignant glioma and neuroglioma cells. B) P69, prostate cancer and HeLa cells. C) FM516 and melanoma cells. GAPDH mRNA was used as loading control.

    Article Snippet: Cell lines and stable clones Normal SV-40-immortalized human prostate epithelial cells (P69) were obtained from Dr. Ware, VCU, and DU-145, PC3 and LNCaP human prostate carcinoma cells were obtained from American Type Culture Collection (ATCC) and cultured as described ( 25 ).

    Techniques: Infection, Purification, Northern Blot, Control

    The indicated cell lines were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell lysates were collected. For detecting protein expression, 50 μg of total protein was run on SDS–PAGE, transferred onto a nitrocellulose membrane and stained with the indicated antibodies, and protein expression was determined by Western blotting in A) Im-PHFA, malignant glioma and neuroglioma, B) P69 and prostate cancer cells, and C) FM516 and melanomas. D) Cells were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell proliferation/viability was assayed by MTT assay. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 cell viability is significantly less than cells infected for 0 h (one way ANOVA with Newman Keuls).

    Journal: Cancer research

    Article Title: Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction

    doi: 10.1158/0008-5472.CAN-13-1062

    Figure Lengend Snippet: The indicated cell lines were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell lysates were collected. For detecting protein expression, 50 μg of total protein was run on SDS–PAGE, transferred onto a nitrocellulose membrane and stained with the indicated antibodies, and protein expression was determined by Western blotting in A) Im-PHFA, malignant glioma and neuroglioma, B) P69 and prostate cancer cells, and C) FM516 and melanomas. D) Cells were infected with Ad.vec or Ad.mda-7 at 100 pfu/cell for the indicated time after which cell proliferation/viability was assayed by MTT assay. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 cell viability is significantly less than cells infected for 0 h (one way ANOVA with Newman Keuls).

    Article Snippet: Cell lines and stable clones Normal SV-40-immortalized human prostate epithelial cells (P69) were obtained from Dr. Ware, VCU, and DU-145, PC3 and LNCaP human prostate carcinoma cells were obtained from American Type Culture Collection (ATCC) and cultured as described ( 25 ).

    Techniques: Infection, Expressing, SDS Page, Membrane, Staining, Western Blot, MTT Assay

    A) SARI full length promoter/pGL3-Luc or SARI deletion mutant promoter/PGL3-Luc constructs were transfected into HeLa cells. Twenty-four h later the cells were treated with IFN-β (1000 U/ml) or infected with Ad.vec (100 pfu/cell) or Ad.mda-7 (100 pfu/cell) for 24 h after which cell lysates were collected for luciferase assays. B) i): HeLa cells were infected with Ad.vec or Ad.SARI at 10 pfu/cell and nuclei were prepared from the treated cells. The isolated nuclei were used to label preinitiated RNA transcription with [α-32P] UTP in vitro, and the purified RNA was hybridized to a dot blot carrying an equivalent amount of a SARI cDNA. The transcription rate of GAPDH served as control. ii) HeLa cells were treated as described in panel A and total RNA was purified, blotted onto a nylon membrane, and Northern blotting was done with a SARI, mda-7/IL-24 or GAPDH cDNA. C) 3′-UTR region of SARI mRNA was cloned into PGL3-basic-Luc vector (Luc-3′UTR) and three independent clones (along with control vector) were transfected into HeLa cells for 24 h followed by infection with Ad.vec or Ad.mda-7 (100 pfu/cell for 24 h) and cell lysates were collected for luciferase assays. The data is presented as the ratio of Ad.mda-7 to Ad.vec treated cells. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 RLU is significantly higher than PGL-3 Luc control cells. D) P69 cells were infected with Ad.vec or Ad.mda-7 (20 pfu/cell for 24 h) after which Actinomycin D (0.5 μg/ml) was added for the indicated times. Total RNA was collected to perform Northern blotting using a SARI cDNA.

    Journal: Cancer research

    Article Title: Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction

    doi: 10.1158/0008-5472.CAN-13-1062

    Figure Lengend Snippet: A) SARI full length promoter/pGL3-Luc or SARI deletion mutant promoter/PGL3-Luc constructs were transfected into HeLa cells. Twenty-four h later the cells were treated with IFN-β (1000 U/ml) or infected with Ad.vec (100 pfu/cell) or Ad.mda-7 (100 pfu/cell) for 24 h after which cell lysates were collected for luciferase assays. B) i): HeLa cells were infected with Ad.vec or Ad.SARI at 10 pfu/cell and nuclei were prepared from the treated cells. The isolated nuclei were used to label preinitiated RNA transcription with [α-32P] UTP in vitro, and the purified RNA was hybridized to a dot blot carrying an equivalent amount of a SARI cDNA. The transcription rate of GAPDH served as control. ii) HeLa cells were treated as described in panel A and total RNA was purified, blotted onto a nylon membrane, and Northern blotting was done with a SARI, mda-7/IL-24 or GAPDH cDNA. C) 3′-UTR region of SARI mRNA was cloned into PGL3-basic-Luc vector (Luc-3′UTR) and three independent clones (along with control vector) were transfected into HeLa cells for 24 h followed by infection with Ad.vec or Ad.mda-7 (100 pfu/cell for 24 h) and cell lysates were collected for luciferase assays. The data is presented as the ratio of Ad.mda-7 to Ad.vec treated cells. All experiments were performed at least three times, and data represent mean ± S.D. * p<0.05 RLU is significantly higher than PGL-3 Luc control cells. D) P69 cells were infected with Ad.vec or Ad.mda-7 (20 pfu/cell for 24 h) after which Actinomycin D (0.5 μg/ml) was added for the indicated times. Total RNA was collected to perform Northern blotting using a SARI cDNA.

    Article Snippet: Cell lines and stable clones Normal SV-40-immortalized human prostate epithelial cells (P69) were obtained from Dr. Ware, VCU, and DU-145, PC3 and LNCaP human prostate carcinoma cells were obtained from American Type Culture Collection (ATCC) and cultured as described ( 25 ).

    Techniques: Mutagenesis, Construct, Transfection, Infection, Luciferase, Isolation, In Vitro, Purification, Dot Blot, Control, Membrane, Northern Blot, Clone Assay, Plasmid Preparation